Journal: Immunity
Article Title: Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation
doi: 10.1016/j.immuni.2023.08.002
Figure Lengend Snippet:
Article Snippet: Cell suspension was subsequently centrifuged, resuspended and a purified Fc-receptor (CD16/32, FcγRIII/II; 93, eBioscience) blocking antibody was added and incubated for 10 min. Primary antibodies against CD11b (#101228; Biolegend®), CD64 (#139304; Biolegend®), F4/80 (#123132, Biolegend®) and a Fixable viability Dye eFluor 780 (#64-0865; eBioscience®) were added and incubated for 20 min. After a final centrifugation step cells were resuspended in a PBS containing 0.5% BSA (FACS buffer).
Techniques: Control, Blocking Assay, Selection, Recombinant, Western Blot, In Situ, Staining, DNA HS Assay, Picogreen Assay, cDNA Synthesis, Clinical Proteomics, SYBR Green Assay, Lysis, Enzyme-linked Immunosorbent Assay, Isolation, Software